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The method used by the OligoScan to obtain results on the amount of trace elements in tissues is the spectrophotometry.

It is a quantitative analytical method, which consists in measuring the absorbance or the optical density of a chemical substance.

The basic principle is that every chemical compound absorbs, emits or reflects light (electromagnetic radiation) over a certain range of wavelength.

The more the sample is concentrated, the more it absorbs the light within the limits of proportionality expressed in the law Beer-Lambert.

The spectrophotometry is used in many domains: chemistry, pharmacy, environment, food-processing industry, biology, medical /clinical, material/chemical engineering and clinical applications and many others.

In the clinical applications, spectrophotometry is used to examine blood or tissues for clinical diagnosis.

absorption example image